Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

I-SceI pB 5UAS::ZfSyGCaMP2
(Plasmid #26125)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 26125 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBluescript II (KS)
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3585
  • Vector type
    Zebrafish expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Synaptophysin GCaMP2
  • Alt name
    SyGCaMP2
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    2262
  • Entrez Gene
    sypb (a.k.a. zgc:136469, zgc:92837)
  • Tag / Fusion Protein
    • GCaMP2 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (destroyed during cloning)
  • 5′ sequencing primer M13-F
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Reference the following (for GCaMP2) when using this plasmid, along with the original article: Tallini YN, Ohkura M, Choi B-R, Ji J, Imoto K, Doran R, Lee J, Plan P, Wilson J, Xin H-B, Sanbe A, Gulick J, Mathai J, Robbins J, Salama G, Nakai J, Kotlikoff MI (2006) Imaging cellular signals in the heart in vivo: Cardiac expression of the high signal Ca2+ indicator G-CaMP2. Proc. Natl. Acad. Sci. USA 103, 4753-4758.
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

At both ends of the multiple cloning site of the original pBluescript II KS+, a I-SceI recognition site was cloned replacing the BssHII cutting sequence.

In the map, ORF frame 3 (1107-3368): zebrafish SyGCaMP2. Also, 5UAS is located 668-1034.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    I-SceI pB 5UAS::ZfSyGCaMP2 was a gift from Leon Lagnado (Addgene plasmid # 26125 ; http://n2t.net/addgene:26125 ; RRID:Addgene_26125)
  • For your References section:

    A genetically encoded reporter of synaptic activity in vivo. Dreosti E, Odermatt B, Dorostkar MM, Lagnado L. Nat Methods. 2009 Dec . 6(12):883-9. 10.1038/nmeth.1399 PubMed 19898484