1
Plasmid 26164: CMV-d2eGFP-empty
  • None

  • 5000

  • …ccgcCTCGAG[sponge sites]GGGCCCgttt… became …ccgcCTCGACgttt…

  • modified pcDNA5
    (Search Vector Database)

  • Mammalian Expression

  • 5000

  • XhoI

  • Yes

  • ApaI

  • Yes

  • CMV-F List of Sequencing Primers

  • BGH-rev

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • View sequences (1)
  • Phil Sharp

  • UBMTA
    Ancillary Agreement for Plasmids Containing FP Materials

Comments: 

This plasmid is to be used ONLY as a negative control for the CMV-d2eGFP plasmids in the associated paper. The multiple cloning site has been destroyed and should not be used for further cloning.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: MicroRNA sponges: competitive inhibitors of small RNAs in mammalian cells. Ebert et al (Nat Methods. 2007 Sep . 4(9):721-6. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26164" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only