Addgene - pdestDD03 Plasmid Data

Plasmid 26414: pdestDD03

Gene/insert name:
attR4-ccdB-attR2 cassette
Insert size:
3479
Vector backbone:
pDP#MM016b
(Search Vector Database)
Vector type:
Bacterial Expression, Worm Expression
Backbone size w/o insert (bp):
8623
5' sequencing primer:
unknown List of Sequencing Primers
Bacterial resistance(s)
Ampicillin and Chloramphenicol
Growth strain(s)
ccdB Survival
Growth temperature (℃):
37
Growth instructions:
ccdB resistant bacteria Ex: DB3.1 E. coli
High or low copy:
High Copy
Sequence: View sequences (4)
Principal Investigator:
Marc Vidal
Terms and Licenses:
MTA

Comments:

A HindIII-KpnI fragment containing the attR4-ccdB-attR2 cassette from pDEST-6 (Invitrogen) was cloned into the unc-119 rescue vector pDP#MM016b.

The unc-119 rescuing fragment present in these vectors can be used as a marker to select transgenic worms.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A first version of the Caenorhabditis elegans Promoterome. Dupuy et al (Genome Res. 2004 Oct;14(10B):2169-75. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26414" in your Materials and Methods section.