pdestDD03
(Plasmid
#26414)
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 26414 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepDP#MM016b
- Backbone size w/o insert (bp) 8623
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Vector typeBacterial Expression, Worm Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Growth instructionsccdB resistant bacteria Ex: DB3.1 E. coli
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameattR4-ccdB-attR2 cassette
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Insert Size (bp)3479
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
A HindIII-KpnI fragment containing the attR4-ccdB-attR2 cassette from pDEST-6 (Invitrogen) was cloned into the unc-119 rescue vector pDP#MM016b.
The unc-119 rescuing fragment present in these vectors can be used as a marker to select transgenic worms.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pdestDD03 was a gift from Marc Vidal (Addgene plasmid # 26414 ; http://n2t.net/addgene:26414 ; RRID:Addgene_26414) -
For your References section:
A first version of the Caenorhabditis elegans Promoterome. Dupuy D, Li QR, Deplancke B, Boxem M, Hao T, Lamesch P, Sequerra R, Bosak S, Doucette-Stamm L, Hope IA, Hill DE, Walhout AJ, Vidal M.. Genome Res. 2004 Oct;14(10B):2169-75. 10.1101/gr.2497604 PubMed 15489340
