Addgene - pdestMB14 Plasmid Data

Plasmid 26415: pdestMB14

Gene/insert name:
attR4-ccdB-attR2
Insert size:
3479
Vector backbone:
pDP#MM016b
(Search Vector Database)
Vector type:
Bacterial Expression, Worm Expression
Backbone size w/o insert (bp):
8623
Cloning site 5':
HindIII
Site destroyed during cloning:
Unknown
Cloning site 3':
KpnI
Site destroyed during cloning:
Unknown
5' sequencing primer:
unknown List of Sequencing Primers
Bacterial resistance(s)
Ampicillin and Chloramphenicol
Growth strain(s)
ccdB Survival
Growth temperature (℃):
37
Growth instructions:
ccdB resistant bacteria Ex: DB3.1 E. coli
High or low copy:
High Copy
Sequence: View sequences (5)
Principal Investigator:
Marc Vidal
Terms and Licenses:
MTA

Comments:

A KpnI-ApaI fragment containing the GFP encoding sequence and the unc-54 3′ UTR from pPD95.79 was cloned into pDP#MM016b. An EcoRV fragment of the attR4-ccdB-attR2 cassette from pDEST-6 (Invitrogen) was cloned into this new construct digested with Acc65I and filled in with Klenow.

The unc-119 rescuing fragment present in these vectors can be used as a marker to select transgenic worms.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A first version of the Caenorhabditis elegans Promoterome. Dupuy et al (Genome Res. 2004 Oct;14(10B):2169-75. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26415" in your Materials and Methods section.