|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26461||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6037
Vector typeMammalian Expression, Bacterial Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)758
/ Fusion Protein
- pEGFP (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site Nde1 (unknown if destroyed)
- 3′ cloning site Xba1 (unknown if destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Articles Citing this Plasmid
Note that there are a few minor discrepancies between Addgene's quality control sequence and the author's sequence, but these should not affect function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEGFP-PCNA-IRES-puro2b was a gift from Daniel Gerlich (Addgene plasmid # 26461 ; http://n2t.net/addgene:26461 ; RRID:Addgene_26461)
For your References section:CellCognition: time-resolved phenotype annotation in high-throughput live cell imaging. Held M, Schmitz MH, Fischer B, Walter T, Neumann B, Olma MH, Peter M, Ellenberg J, Gerlich DW. Nat Methods. 2010 Sep . 7(9):747-54. 10.1038/nmeth.1486 PubMed 20693996