Addgene - pLenti CMVtight eGFP Blast (w782-1) Plasmid Data

Plasmid 26584: pLenti CMVtight eGFP Blast (w782-1)

Gene/insert name:
Enhanced Green Fluorescent Protein
Alt name:
eGFP
Insert size:
719
Vector backbone:
p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral ; Tet-On Advanced vector
Backbone size w/o insert (bp):
7580
Cloning site 5':
Bst BI
Site destroyed during cloning:
No
Cloning site 3':
Xba I
Site destroyed during cloning:
No
5' sequencing primer:
CMVtightFORW List of Sequencing Primers
3' sequencing primer:
WPRErev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
Stbl3, 37oC
High or low copy:
High Copy
Selectable markers:
Blasticidin
Person or lab that originally
cloned the gene/insert:
The CMVtight element was a gift from Dr. Dominic Esposito.
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Eric Campeau
Terms and Licenses:
MTA
Tet Systems Limited Use Label License

Comments:

Need to be transduced in a Tet-On Advanced cell line for expression of the GFP.

There are a few mismatches between Addgene's sequence and Dr Campeau's sequence. The sequence difference does not affect plasmid function.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26584" in your Materials and Methods section.