pLenti CMVtight eGFP Blast (w782-1)
Vector backbonep156RRL-sinPPT-CMV-GFP-PRE/Nhe I
- Backbone size w/o insert (bp) 7580
Vector typeMammalian Expression, Lentiviral ; Tet-On Advanced vector
Growth in Bacteria
Growth instructionsStbl3, 37oC
Copy numberHigh Copy
Gene/Insert nameEnhanced Green Fluorescent Protein
Insert Size (bp)719
- Cloning method Restriction Enzyme
- 5′ cloning site Bst BI (not destroyed)
- 3′ cloning site Xba I (not destroyed)
- 5′ sequencing primer CMVtightFORW
- 3′ sequencing primer WPRErev (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byThe CMVtight element was a gift from Dr. Dominic Esposito.
Terms and Licenses
Need to be transduced in a Tet-On Advanced cell line for expression of the GFP.
There are a few mismatches between Addgene's sequence and Dr Campeau's sequence. The sequence difference does not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLenti CMVtight eGFP Blast (w782-1) was a gift from Eric Campeau (Addgene plasmid # 26584)
Map generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.