pLenti CMVtight eGFP Puro (w771-1)
(Plasmid #26431)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
  • Backbone size w/o insert (bp) 7866
  • Vector type
    Mammalian Expression, Lentiviral ; Tet-On Advanced vector
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Stbl3, 37oC.
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    Enhanced Green Fluorescent Protein
  • Alt name
    eGFP
  • Insert Size (bp)
    719

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bst BI (not destroyed)
  • 3′ cloning site Xba I (not destroyed)
  • 5′ sequencing primer CMVtightFORW
  • 3′ sequencing primer WPRErev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Need to be transduced in a Tet-On Advanced cell line for expression of GFP.

There are a few mismatches between Addgene's sequence and Dr Campeau's sequence. The sequence differences do not affect GFP expression or general plasmid function.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti CMVtight eGFP Puro (w771-1) was a gift from Eric Campeau (Addgene plasmid # 26431)