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Plasmid 26431: pLenti CMVtight eGFP Puro (w771-1)
  • Enhanced Green Fluorescent Protein

  • eGFP

  • 719

  • p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
    (Search Vector Database)

  • Mammalian Expression, Lentiviral ; Tet-On Advanced vector

  • 7866

  • Bst BI

  • No

  • Xba I

  • No

  • CMVtightFORW List of Sequencing Primers

  • WPRErev

  • Ampicillin

  • Stbl3

  • 37

  • Stbl3, 37oC.

  • High Copy

  • Puromycin

  • The CMVtight element was a gift from Dr. Dominic Esposito.

  • View sequences (2)
  • View map

  • Eric Campeau

  • UBMTA
    Ancillary Agreement for Plasmids Containing FP Materials

Comments: 

Need to be transduced in a Tet-On Advanced cell line for expression of GFP.

There are a few mismatches between Addgene's sequence and Dr Campeau's sequence. The sequence differences do not affect GFP expression or general plasmid function.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26431" in your Materials and Methods section.