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Plasmid 26431: pLenti CMVtight eGFP Puro (w771-1)
  • Enhanced Green Fluorescent Protein

  • eGFP

  • 719

  • p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
    (Search Vector Database)

  • Mammalian Expression, Lentiviral ; Tet-On Advanced vector

  • 7866

  • Bst BI

  • No

  • Xba I

  • No

  • CMVtightFORW List of Sequencing Primers

  • WPRErev

  • Ampicillin

  • Stbl3

  • 37

  • Stbl3, 37oC.

  • High Copy

  • Puromycin

  • The CMVtight element was a gift from Dr. Dominic Esposito.

  • View sequences (2)
  • View map

  • Eric Campeau

  • UBMTA
    Ancillary Agreement for Plasmids Containing FP Materials

Comments: 

Need to be transduced in a Tet-On Advanced cell line for expression of GFP.

There are a few mismatches between Addgene's sequence and Dr Campeau's sequence. The sequence differences do not affect GFP expression or general plasmid function.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26431" in your Materials and Methods section.