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Nanog-luciferase targeting construct
(Plasmid #26591)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 26591 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSP72
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 2394
  • Vector type
    Bacterial Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nanog promoter-luciferase-lox-pgk-neomycin-loc
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    6331

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Nanog-luciferase targeting construct was a gift from Rudolf Jaenisch (Addgene plasmid # 26591)
  • For your References section:

    Reprogramming of murine fibroblasts to induced pluripotent stem cells with chemical complementation of Klf4. Lyssiotis CA, Foreman RK, Staerk J, Garcia M, Mathur D, Markoulaki S, Hanna J, Lairson LL, Charette BD, Bouchez LC, Bollong M, Kunick C, Brinker A, Cho CY, Schultz PG, Jaenisch R. Proc Natl Acad Sci U S A. 2009 Jun 2. 106(22):8912-7. 10.1073/pnas.0903860106 PubMed 19447925