Addgene - pGL2-hOct4 DE-SV40-Luc Plasmid Data

Plasmid 26279: pGL2-hOct4 DE-SV40-Luc

Gene/insert name:
Oct4 Distal Enhancer Element (DE)
Insert size:
2002
Species:
H. sapiens (human)
Vector backbone:
pGL2-SV40-Luc
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5000
Cloning site 5':
XhoI
Site destroyed during cloning:
No
Cloning site 3':
KpnI
Site destroyed during cloning:
No
5' sequencing primer:
ggtacccattgagtccaaatcct List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
Backbone kindly provided from Dr. Ron Mckay and Dr. Paul Tesar
Sequence: View sequences (2)
Principal Investigator:
Rudolf Jaenisch
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Human embryonic stem cells with biological and epigenetic characteristics similar to those of mouse ESCs. Hanna et al (Proc Natl Acad Sci U S A. 2010 May 18. 107(20):9222-7. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26279" in your Materials and Methods section.