Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26611||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5400
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameeEF2 5'UTR
Alt namefirefly luciferase
Insert Size (bp)1732
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
eEF2 5' UTR was amplified from total RNA.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3-TOP luciferase was a gift from Jie Chen (Addgene plasmid # 26611 ; http://n2t.net/addgene:26611 ; RRID:Addgene_26611)
For your References section:Cytoplasmic-nuclear shuttling of FKBP12-rapamycin-associated protein is involved in rapamycin-sensitive signaling and translation initiation. Kim JE, Chen J. Proc Natl Acad Sci U S A. 2000 Dec 19. 97(26):14340-5. 10.1073/pnas.011511898 PubMed 11114166