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pCAG-Cre
(Plasmid #26647)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 26647 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCAG
  • Backbone size w/o insert (bp) 4800
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cre
  • Alt name
    Cre-recombinase
  • Species
    Bacteriophage P1
  • Insert Size (bp)
    1000

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SmaI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pCAG-Fwd
  • 3′ sequencing primer Bglob-pA-R
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Cre was excised from pcDNA3Cre as a directionaly sticy blunt by first cutting with HindIII, then blunting with Klenow, and finally cutting with NotI. The directional Cre fragment was cloned into the pCAG backbone. The eGFP cassette was removed from the pCAG-GPF backbone using SmaI (which left a blunt end) and NotI. Cre was then subcloned directionally into the pCAG backbone.

This construct does NOT express GFP.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-Cre was a gift from Anjen Chenn (Addgene plasmid # 26647 ; http://n2t.net/addgene:26647 ; RRID:Addgene_26647)