|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26647||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4800
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)1000
- Cloning method Restriction Enzyme
- 5′ cloning site SmaI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer pCAG-Fwd
- 3′ sequencing primer Bglob-pA-R (Common Sequencing Primers)
Cre was excised from pcDNA3Cre as a directionaly sticy blunt by first cutting with HindIII, then blunting with Klenow, and finally cutting with NotI. The directional Cre fragment was cloned into the pCAG backbone. The eGFP cassette was removed from the pCAG-GPF backbone using SmaI (which left a blunt end) and NotI. Cre was then subcloned directionally into the pCAG backbone.
This construct does NOT express GFP.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCAG-Cre was a gift from Anjen Chenn (Addgene plasmid # 26647 ; http://n2t.net/addgene:26647 ; RRID:Addgene_26647)