|Item||Catalog #||Description||Quantity||Price (USD)|
|Bacterial Strain||26930||Bacteria in agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeE.coli bacterial strain
Growth in Bacteria
Growth instructionsGrow at 30oC
- Cloning method Restriction Enzyme
- 5′ sequencing primer N/A (Common Sequencing Primers)
MG1655, bla, bio-, lambda-Red1
From the paper: Lambda prophage (from strain DY330; Yu et al. (2000), PNAS 97: 5978-5983) was modified to include the bla gene and introduced into wild-type MG1655 E.coli by P1 transduction at the bioA/bioB gene locus and selected on ampicillin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:EcNR1 Strain was a gift from George Church (Addgene plasmid # 26930)
For your References section:Programming cells by multiplex genome engineering and accelerated evolution. Wang HH, Isaacs FJ, Carr PA, Sun ZZ, Xu G, Forest CR, Church GM. Nature. 2009 Aug 13. 460(7257):894-8. 10.1038/nature08187 PubMed 19633652