|Item||Catalog #||Description||Quantity||Price (USD)|
|Bacterial Strain||41700||Bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Growth in Bacteria
Growth instructionsDo not grow above 34 C because lambda red will begin to have leaky expression.
Copy numberLow Copy
Gene/Insert nameE. coli MG1655 ΔmutS::cat Δ(ybhB-bioAB)::[λcI857 N(cro-ea59)::tetR-bla] dnaG.Q576A
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:EcNR2.dnaG.Q576A was a gift from George Church (Addgene plasmid # 41700)
For your References section:Manipulating replisome dynamics to enhance lambda Red-mediated multiplex genome engineering. Lajoie MJ, Gregg CJ, Mosberg JA, Washington GC, Church GM. Nucleic Acids Res. 2012 Aug 16. 10.1093/nar/gks751 PubMed 22904085