1
Plasmid 27092: pRS3 (CK2alpha, CK2beta)
  • CK2beta

  • CSNK2B, G5A, CK2β, CK2N, CSK2B, MGC138222, MGC138224

  • Casein Kinase II beta

  • H. sapiens (human)

  • CSNK2B (DADB-127H9.2, CK2B, CK2N, CSK2B, G5A)

  • Myc

  • N terminal on backbone

  • CK2alpha

  • CSNK2A1, CKII, CK2A1

  • CK2α

  • Casein Kinase II alpha

  • H. sapiens (human)

  • CSNK2A1 (CK2A1, CKII, CSNK2A3)

  • HA

  • C terminal on insert

  • pBI
    (Search Vector Database)

  • Clontech

  • Mammalian Expression ; tetracycline-regulated expression

  • 4358

  • bidirectional tet-responsive promoter

  • HindIII

  • No

  • NsiI

  • No

  • NA List of Sequencing Primers

  • EBV-rev

  • bidirectional tet-responsive promoter

  • MluI

  • No

  • ApaI

  • No

  • NA List of Sequencing Primers

  • Bglob-intron-R

  • Ampicillin

  • DH5alpha

  • 37

  • Low Copy

  • View sequences (3)
  • David Litchfield

  • UBMTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Protein kinase CK2 is a constitutively active enzyme that promotes cell survival: strategies to identify CK2 substrates and manipulate its activity in mammalian cells. Turowec et al (Methods Enzymol. 2010;484:471-93. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 27092" in your Materials and Methods section.