Gene/Insert 1
Gene/insert name: CK2beta
Alt name: CSNK2B, G5A, CK2β, CK2N, CSK2B, MGC138222, MGC138224
Alt name: Casein Kinase II beta
Species:
H. sapiens (human)
Entrez Gene:
CSNK2B
(DADB-127H9.2, CK2B, CK2N, CSK2B, G5A)
Fusion protein or tag: Myc
Terminal: N terminal on backbone
Gene/Insert 2
Gene/insert name: CK2alpha
Alt name: CSNK2A1, CKII, CK2A1
Alt name: CK2α
Alt name: Casein Kinase II alpha
Species:
H. sapiens (human)
Entrez Gene:
CSNK2A1
(CK2A1, CKII, CSNK2A3)
Fusion protein or tag: HA
Terminal: C terminal on insert
Vector backbone:
pBISearch Vector Database )
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression ; tetracycline-regulated expression
Backbone size w/o insert (bp):
4358
Cloning information for Gene/Insert 1
Promoter: bidirectional tet-responsive promoter
Cloning site 5':
HindIII
Site destroyed during cloning:
No
Cloning site 3':
NsiI
Site destroyed during cloning:
No
5' sequencing primer:
NA
List of Sequencing Primers
3' sequencing primer:
EBV-rev
Cloning information for Gene/Insert 2
Promoter: bidirectional tet-responsive promoter
Cloning site 5':
MluI
Site destroyed during cloning:
No
Cloning site 3':
ApaI
Site destroyed during cloning:
No
5' sequencing primer:
NA
List of Sequencing Primers
3' sequencing primer:
Bglob-intron-R
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Low Copy
Sequence:
View sequences (3)
Principal Investigator:
David Litchfield
Terms and Licenses:
MTA
Addgene has sequenced a portion of this plasmid for verification.
Click here for the sequencing
result.
Please acknowledge the principal investigator and cite this article if you use
this plasmid in a publication. Also, please include the text "Addgene plasmid
27092" in your Materials and Methods section.
