Addgene - pRS2 (CK2alpha', CK2beta) Plasmid Data

Plasmid 27093: pRS2 (CK2alpha', CK2beta)

Gene/Insert 1
Gene/insert name:
CK2beta
Alt name:
CSNK2B, G5A, CK2β, CK2N, CSK2B, MGC138222, MGC138224
Alt name:
Casein Kinase II beta
Species:
H. sapiens (human)
Entrez Gene:
CSNK2B (DADB-127H9.2, CK2B, CK2N, CSK2B, G5A)
Fusion protein or tag:
Myc
Terminal:
N terminal on insert
Gene/Insert 2
Gene/insert name:
CK2alpha'
Alt name:
CK2A2; CSNK2A1; FLJ43934
Alt name:
CK2α'
Alt name:
Casein Kinase II alpha prime
Species:
H. sapiens (human)
Entrez Gene:
CSNK2A2 (CK2A2, CSNK2A1)
Fusion protein or tag:
HA
Terminal:
N terminal on backbone
Vector backbone:
pBI
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression ; tetracycline-regulated expression
Backbone size w/o insert (bp):
4358
Cloning information for Gene/Insert 1
Promoter:
bidirectional tet-responsive promoter
Cloning site 5':
MluI
Site destroyed during cloning:
No
Cloning site 3':
ApaI
Site destroyed during cloning:
Unknown
5' sequencing primer:
NA List of Sequencing Primers
3' sequencing primer:
Bglob-intron-R
Cloning information for Gene/Insert 2
Promoter:
bidirectional tet-responsive promoter
Cloning site 5':
HindIII
Site destroyed during cloning:
No
Cloning site 3':
HindIII
Site destroyed during cloning:
No
5' sequencing primer:
NA List of Sequencing Primers
3' sequencing primer:
EBV-rev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Low Copy
Sequence: View sequences (4)
Principal Investigator:
David Litchfield
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Protein kinase CK2 is a constitutively active enzyme that promotes cell survival: strategies to identify CK2 substrates and manipulate its activity in mammalian cells. Turowec et al (Methods Enzymol. 2010;484:471-93. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 27093" in your Materials and Methods section.