pEnt L1L3 rtTA-3
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||27106||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2300
Vector typeGateway entry vector
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namereverse tetracycline transactivator
Insert Size (bp)1100
- Cloning method Gateway Cloning
- 5′ sequencing primer TCTACAAACTCTTCCTGTTAGT
- 3′ sequencing primer AGAGATTTTGAGACACGGGC (Common Sequencing Primers)
Terms and Licenses
Gateway L1L3 entry vector containing the rtTA regulator, rtTA2s-M2, an optimized version of the rtTA transactivator (see PubMedID 10859354 for details). Promoters can be inserted using PacI and SbfI. Does not contain insulator sequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEnt L1L3 rtTA-3 was a gift from Edward Hsiao (Addgene plasmid # 27106 ; http://n2t.net/addgene:27106 ; RRID:Addgene_27106)
For your References section:Constitutive Gs activation using a single-construct tetracycline-inducible expression system in embryonic stem cells and mice. Hsiao EC, Nguyen TD, Ng JK, Scott MJ, Chang WC, Zahed H, Conklin BR. Stem Cell Res Ther. 2011 Mar 4. 2(2):11. 10.1186/scrt52 PubMed 21375737