|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||19407||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 2932
Modifications to backboneLow-copy E. coli origin of pTREtight was exchanged with the high-copy pUC origin
Vector typeMammalian Expression
Growth in Bacteria
Growth instructionsDH5a at 37°C
Copy numberHigh Copy
Gene/Insert nameempty vector
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer T7 Term (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pTREtight2 was a gift from Markus Ralser (Addgene plasmid # 19407 ; http://n2t.net/addgene:19407 ; RRID:Addgene_19407)