pcDNA-FLAG-NEMO-C54/347A
(Plasmid #27268)

• Depositing Lab: Thomas Gilmore
• Publication: Herscovitch et al Biochem Biophys Res Commun. 2008 Feb 29. 367(1):103-8.

Backbone

• Vector backbone: pcDNA
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NEMO
• Species: H. sapiens (human)
• Insert Size (bp): 1254
• Mutation: Cys54Ala Cys347Ala
• Entrez Gene: IKBKG (a.k.a. AMCBX1, EDAID1, FIP-3, FIP3, Fip3p, IKK-gamma, IKKAP1, IKKG, IMD33, IP, IP1, IP2, IPD2, NEMO, SAIDX, ZC2HC9)
• Tag / Fusion Protein:
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH-Rev

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

NEMO cDNA starts at aa 2.