myrAkt delta4-129
(Plasmid #10841)

• Depositing Lab: Richard Roth
• Publication: Kohn et al J Biol Chem. 1996 Sep 6. 271(36):21920-6.

Backbone

• Vector backbone: pECE
• Backbone manufacturer: William Rutter
• Backbone size w/o insert (bp): 2924
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Akt
• Alt name: myr Akt deltaPH
• Species: H. sapiens (human)
• Insert Size (bp): 1500
• Mutation: Deletion of PH domain (aa4-129)
• Entrez Gene: AKT1 (a.k.a. AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA)
• Promoter: SV40
• Tags / Fusion Proteins:
  • myr (N terminal on insert)
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: XbaI (unknown if destroyed)
• 5′ sequencing primer: SV40pro-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• Articles Citing this Plasmid:
  • 33 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Constitutively active Akt. 14 aa src myristoylation signal sequence fused to N terminus of Akt delta4-129.

Note: Addgene NGS results detected an 8 bp deletion in the 5' end of the SV40 promoter. Plasmid should function as described in the associated publication.