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myrAkt delta4-129
(Plasmid #10841)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 10841 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pECE
  • Backbone manufacturer
    William Rutter
  • Backbone size w/o insert (bp) 2924
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Akt
  • Alt name
    myr Akt deltaPH
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1500
  • Mutation
    Deletion of PH domain (aa4-129)
  • Entrez Gene
    AKT1 (a.k.a. AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA)
  • Promoter SV40
  • Tags / Fusion Proteins
    • myr (N terminal on insert)
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer SV40pro-F
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Constitutively active Akt. 14 aa src myristoylation signal sequence fused to N terminus of Akt delta4-129.

Note: Addgene NGS results detected an 8 bp deletion in the 5' end of the SV40 promoter. Plasmid should function as described in the associated publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    myrAkt delta4-129 was a gift from Richard Roth (Addgene plasmid # 10841 ; http://n2t.net/addgene:10841 ; RRID:Addgene_10841)
  • For your References section:

    Akt, a pleckstrin homology domain containing kinase, is activated primarily by phosphorylation. Kohn AD, Takeuchi F, Roth RA. J Biol Chem. 1996 Sep 6. 271(36):21920-6. 10.1074/jbc.271.36.21920 PubMed 8702995