|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||10841||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerWilliam Rutter
- Backbone size w/o insert (bp) 2924
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Alt namemyr Akt deltaPH
SpeciesH. sapiens (human)
Insert Size (bp)1500
MutationDeletion of PH domain (aa4-129)
Entrez GeneAKT1 (a.k.a. AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA)
- Promoter SV40
/ Fusion Proteins
- myr (N terminal on insert)
- HA (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site XbaI (unknown if destroyed)
- 5′ sequencing primer SV40pro-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Constitutively active Akt. 14 aa src myristoylation signal sequence fused to N terminus of Akt delta4-129.
Note: Addgene NGS results detected an 8 bp deletion in the 5' end of the SV40 promoter. Plasmid should function as described in the associated publication.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:myrAkt delta4-129 was a gift from Richard Roth (Addgene plasmid # 10841 ; http://n2t.net/addgene:10841 ; RRID:Addgene_10841)
For your References section:Akt, a pleckstrin homology domain containing kinase, is activated primarily by phosphorylation. Kohn AD, Takeuchi F, Roth RA. J Biol Chem. 1996 Sep 6. 271(36):21920-6. 10.1074/jbc.271.36.21920 PubMed 8702995