Addgene - myrAkt delta4-129 Plasmid Data

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10841: myrAkt delta…
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Plasmid 10841: myrAkt delta4-129

Gene/insert name:
Akt
Alt name:
myr Akt deltaPH
Insert size:
1500
Species:
H. sapiens (human)
Entrez Gene:
AKT1 (AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA)
Fusion protein or tag:
myr
Terminal:
N terminal on insert
Fusion protein or tag:
HA
Terminal:
C terminal on insert
Mutation:
Deletion of PH domain (aa4-129)
Vector backbone:
pECE
(Search Vector Database)
Backbone manufacturer:
William Rutter
Vector type:
Mammalian Expression
Backbone size w/o insert:
2924
5' sequencing primer:
SV40pro-F List of Sequencing Primers
Bacterial resistance:
Ampicillin
Growth strain:
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Richard Roth
Terms and Licenses:
MTA

Comments:

Constitutively active Akt. 14 aa src myristoylation signal sequence fused to N terminus of Akt delta4-129.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Akt, a pleckstrin homology domain containing kinase, is activated primarily by phosphorylation. Kohn et al (J Biol Chem. 1996 Sep 6. 271(36):21920-6. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 10841" in your Materials and Methods section.