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LeGO-iG2
(Plasmid #27341)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 27341 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    LeGO
  • Backbone size w/o insert (bp) 7829
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Any (like TOP10, XL10-Gold or Stbl)
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SFFV promoter, IRES-eGFP

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The eGFP cDNA is from Clontech. The lenti backbone is a derivative of pLentiLox3.7 developed at the MIT (http://web.mit.edu/jacks-lab/protocols/pll37.htm).
  • Articles Citing this Plasmid

Terms and Licenses

Depositor Comments

HIV-1 derived third generation lentiviral vector for the expression of a cDNA cloned into the multiple cloning site (BamHI, EcoRI, SbfI, StuI, NotI). An internal ribosome entry site (IRES) is used to express the marker gene. Use second generation (like psPAX2) or third generation (like pMDLg/pRRE + pRSV-Rev) systems for packaging in addition to a VSV-G expressing plasmid (or another envelope protein). Please visit the LeGO-Vector home page for more information: http://www.LentiGO-Vectors.de

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LeGO-iG2 was a gift from Boris Fehse (Addgene plasmid # 27341 ; http://n2t.net/addgene:27341 ; RRID:Addgene_27341)
  • For your References section:

    A multicolor panel of novel lentiviral "gene ontology" (LeGO) vectors for functional gene analysis. Weber K, Bartsch U, Stocking C, Fehse B. Mol Ther. 2008 Apr . 16(4):698-706. 10.1038/mt.2008.6 PubMed 18362927