Addgene - plenti6.3/hCx43-stop Plasmid Data

Plasmid 27383: plenti6.3/hCx43-stop

Gene/insert name:
Gja1
Alt name:
Connexin 43
Insert size:
1149
Species:
H. sapiens (human)
GenBank ID:
NM_000165
Entrez Gene:
GJA1 (AVSD3, CX43, DFNB38, GJAL, HLHS1, HSS, ODDD)
Vector backbone:
plenti6.3/V5-DEST
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral
Backbone size w/o insert (bp):
9387
Cloning method:
Gateway Cloning
Cloning site 5':
attR1 gateway reaction site
Site destroyed during cloning:
No
Cloning site 3':
attR2 gateway reaction site
Site destroyed during cloning:
No
5' sequencing primer:
sequence List of Sequencing Primers
3' sequencing primer:
sequence
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Blasticidin
Person or lab that originally
cloned the gene/insert:
Open Biosystems
Sequence: View sequences (1)
Principal Investigator:
Robin Shaw
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Limited forward trafficking of connexin 43 reduces cell-cell coupling in stressed human and mouse myocardium. Smyth et al (J Clin Invest. 2010 Jan 4. 120(1):266-79. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 27383" in your Materials and Methods section.