Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||31807||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2600
Vector typeMammalian Expression
Growth in Bacteria
Copy numberLow Copy
SpeciesH. sapiens (human)
Entrez GeneGJA1 (a.k.a. AVSD3, CMDR, CX43, EKVP, EKVP3, GJAL, HLHS1, HSS, ODDD, PPKCA)
- Promoter P-TIGHT
/ Fusion Protein
- eYFP (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer LNCX_primer
- 3′ sequencing primer EXFP-R (Common Sequencing Primers)
Terms and Licenses
Articles Citing this Plasmid
Human Cx43 was initially cloned into pEYFP-N1 using the NheI and HindIII cut sites of this vector. Cx43-EYFP was then excised from pEYFP-N1 using NheI and NotI to insert the fusion protein encoding genes into pTRE-Tight, so importantly, some of the MCS from pEYFP-N1 remains in the final construct.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pTRE-TIGHT-Cx43-eYFP was a gift from Robin Shaw (Addgene plasmid # 31807 ; http://n2t.net/addgene:31807 ; RRID:Addgene_31807)
For your References section:Limited forward trafficking of connexin 43 reduces cell-cell coupling in stressed human and mouse myocardium. Smyth JW, Hong TT, Gao D, Vogan JM, Jensen BC, Fong TS, Simpson PC, Stainier DY, Chi NC, Shaw RM. J Clin Invest. 2010 Jan 4. 120(1):266-79. 10.1172/JCI39740 PubMed 20038810