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p2.1 (pAGL2A)
(Plasmid #27563)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 27563 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL2-Promoter
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5789
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ENO1 promoter
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    68
  • Entrez Gene
    ENO1 (a.k.a. ENO1L1, HEL-S-17, MPB1, NNE, PPH)
  • Tag / Fusion Protein
    • Luciferase (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer LucNRev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

68-bp sequence from the ENO1 promoter (nt -416 to -347).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p2.1 (pAGL2A) was a gift from Gregg Semenza (Addgene plasmid # 27563 ; http://n2t.net/addgene:27563 ; RRID:Addgene_27563)
  • For your References section:

    Hypoxia response elements in the aldolase A, enolase 1, and lactate dehydrogenase A gene promoters contain essential binding sites for hypoxia-inducible factor 1. Semenza GL, Jiang BH, Leung SW, Passantino R, Concordet JP, Maire P, Giallongo A. J Biol Chem. 1996 Dec 20. 271(51):32529-37. 10.1074/jbc.271.51.32529 PubMed 8955077