pLenti CMVTRE3G eGFP Blast (w818-1)
Vector backbonep156RRL-sinPPT-CMV-GFP-PRE/Nhe I
- Backbone size w/o insert (bp) 7620
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Growth instructionsStbl3, 37oC
Copy numberHigh Copy
Gene/Insert nameEnhance Green Fluorescent Protein
Insert Size (bp)719
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMVtight
- 3′ sequencing primer WPRErev (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byThe CMVTRE3G promoter was a gift from Dr. Dominic Esposito (SAIC Frederick). Tet-On 3G compatible plasmid.
Terms and Licenses
There are a few mismatches between Addgene's quality control sequence and depositor's reference sequence. However the changes are in vector sequence only, features unaffected. Depositor tested this construct and it was functional.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLenti CMVTRE3G eGFP Blast (w818-1) was a gift from Eric Campeau (Addgene plasmid # 27568)
Map generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.