Gene/insert name: USP08
Alt name: PDB: 3N3K
Alt name: ubiquitin specific protease 8
Insert size: 1131
Species:
H. sapiens (human)
Entrez Gene:
USP8
(HumORF8, UBPY)
Fusion protein or tag: His tag with TEV site
Terminal: N terminal on backbone
Mutation: Catalytic domain only.
Vector backbone:
pET28a-LIC (Addgene plasmid 26094)Search Vector Database )
Backbone manufacturer:
SGC
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
7328
Cloning site 5':
Ligation-independent cloning
Site destroyed during cloning:
Unknown
Cloning site 3':
Ligation-independent cloning
Site destroyed during cloning:
Unknown
5' sequencing primer:
T7
List of Sequencing Primers
3' sequencing primer:
T7-term
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence:
View sequences (2)
Principal Investigator:
Cheryl Arrowsmith
Terms and Licenses:
MTA
EMD Millipore Limited Use Label License/Brookhaven pET Assurance Letter
Addgene has sequenced a portion of this plasmid for verification.
Click here for the sequencing
result.
Please acknowledge the principal investigator and cite this article if you use
this plasmid in a publication. Also, please include the text "Addgene plasmid
28115" in your Materials and Methods section.
PDB: 3N3K. http://www.thesgc.org/structures/structure_desc...
This construct is identical to 2GFO