EPHB3
(Plasmid #28165)

• Purpose: Bacterial expression for structure determination; may not be full ORF
• Depositing Lab: Cheryl Arrowsmith
• Publication: SGC Q3 (unpublished)

Backbone

• Vector backbone: pFHMSP-LIC-N (Addgene plasmid 26095)
• Backbone manufacturer: SGC
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EPHB3
• Alt name: PDB: 3P1I
• Alt name: EPH receptor B3, ligand binding domain
• Species: H. sapiens (human)
• Insert Size (bp): 519
• Mutation: Ligand binding domain (residues 39-211).
• Entrez Gene: EPHB3 (a.k.a. EK2, ETK2, HEK2, TYRO6)
• Tags / Fusion Proteins:
  • Honeybee melittin Signal sequence (N terminal on backbone)
  • His tag with TEV cleavage (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ligation-independent cloning (unknown if destroyed)
• 3′ cloning site: ligation-independent cloning (unknown if destroyed)
• 5′ sequencing primer: polyhedrin-fwd

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

PDB: 3P1I. http://www.thesgc.org/structures/3P1I/