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DECIPHER Lentiviral shRNA Library, Mouse Mod 1: Pathway Targets
(Pooled library #28287)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pRSI9-U6-(sh)-UbiC-TagRFP-2A-Puro
  • Backbone manufacturer
    Cellecta , Available from Addgene (#28289)
  • Backbone size w/o insert (bp) 7500
  • Vector type
    Mammalian Expression, Lentiviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NA
  • Growth instructions
    Plasmids need to be grown in OmniMAX 2 T1R (Life Technologies) or SURE (Agilent) Recombination defective E.coli cells
  • Copy number
    Unknown

Sequence Information

  • Depositor Sequences
    None.
  • Addgene Sequences

Full plasmid sequence is available only if provided by the depositing laboratory.

Depositor Comments

Library is supplied as 120μl of DNA at 1.0μg/μl

DECIPHER libraries are bar-coded lentiviral shRNA libraries optimized for RNAi Genetic Screens in pooled format. The first mouse module is intended for the widest possible screening applications. It consists of the following gene groups:

* Members of the major canonical and non-canonical pathways, covered by KEGG, Reactome, and other expert-curated pathway databases

* Genes on the CSHL Cancer 1000 list and 2500 top-ranking genes from the Cancer Genome Atlas, with priority based on the involvement in canonical pathways and disease association

* Approximately 900 genes with gene products that can be targeted by the FDA-approved small molecule compounds

* Most important genes involved in top MeSH ranking diseases; these are chosen by their position within the pathway and number of nodes of connectivity

Thus, Module 1 is both versatile and wide in scope.

PLEASE NOTE: The DECIPHER shRNA are designed for target mRNA associated with the RefSeq#, not the HUGO gene symbol. We can't guarantee that the gene symbols indicated are accurate, as there may be synonyms, nomenclature updates/changes, or other discrepancies in naming that are beyond our control. Please see the HUGO Gene Nomenclature Committee website at http://www.genenames.org/hgnc-guidelines for more information.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DECIPHER Lentiviral shRNA Library, Mouse Mod 1: Pathway Targets was a gift from Alex Chenchik & Gus Frangou (Addgene plasmid # 28287)