Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at Learn more

DECIPHER Lentiviral shRNA Library, Mouse Mod 1: Pathway Targets
(Pooled library #28287)

Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Pooled Library 28287 Shipped as suspended DNA in a microcentrifuge tube 1 $450 Add to Cart
Available to Academic and Nonprofits Only


  • Vector backbone
  • Backbone manufacturer
    Cellecta , Available from Addgene (#28289)
  • Backbone size w/o insert (bp) 7500
  • Vector type
    Mammalian Expression, Lentiviral, RNAi
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    Plasmids need to be grown in OmniMAX 2 T1R (Life Technologies) or SURE (Agilent) Recombination defective E.coli cells
  • Copy number

Depositor Comments

Library is supplied as 120μl of DNA at 1.0μg/μl

DECIPHER libraries are bar-coded lentiviral shRNA libraries optimized for RNAi Genetic Screens in pooled format. The first mouse module is intended for the widest possible screening applications. It consists of the following gene groups:

* Members of the major canonical and non-canonical pathways, covered by KEGG, Reactome, and other expert-curated pathway databases

* Genes on the CSHL Cancer 1000 list and 2500 top-ranking genes from the Cancer Genome Atlas, with priority based on the involvement in canonical pathways and disease association

* Approximately 900 genes with gene products that can be targeted by the FDA-approved small molecule compounds

* Most important genes involved in top MeSH ranking diseases; these are chosen by their position within the pathway and number of nodes of connectivity

Thus, Module 1 is both versatile and wide in scope.

PLEASE NOTE: The DECIPHER shRNA are designed for target mRNA associated with the RefSeq#, not the HUGO gene symbol. We can't guarantee that the gene symbols indicated are accurate, as there may be synonyms, nomenclature updates/changes, or other discrepancies in naming that are beyond our control. Please see the HUGO Gene Nomenclature Committee website at for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DECIPHER Lentiviral shRNA Library, Mouse Mod 1: Pathway Targets was a gift from Alex Chenchik & Gus Frangou (Addgene plasmid # 28287)