Pooled Library 28287: DECIPHER Lentiviral shRNA Library, Mouse Mod 1: Pathway Targets

Library is supplied as 120μl of DNA at 1.0μg/μl

DECIPHER libraries are bar-coded lentiviral shRNA libraries optimized for RNAi Genetic Screens in pooled format. The first mouse module is intended for the widest possible screening applications. It consists of the following gene groups:

* Members of the major canonical and non-canonical pathways, covered by KEGG, Reactome, and other expert-curated pathway databases

* Genes on the CSHL Cancer 1000 list and 2500 top-ranking genes from the Cancer Genome Atlas, with priority based on the involvement in canonical pathways and disease association

* Approximately 900 genes with gene products that can be targeted by the FDA-approved small molecule compounds

* Most important genes involved in top MeSH ranking diseases; these are chosen by their position within the pathway and number of nodes of connectivity

Thus, Module 1 is both versatile and wide in scope.

PLEASE NOTE: The DECIPHER shRNA are designed for target mRNA associated with the RefSeq#, not the HUGO gene symbol. We can't guarantee that the gene symbols indicated are accurate, as there may be synonyms, nomenclature updates/changes, or other discrepancies in naming that are beyond our control. Please see the HUGO Gene Nomenclature Committee website at http://www.genenames.org/hgnc-guidelines for more information.

This plasmid has not been sequence verified. Please contact us if you would like Addgene to sequence a portion of this plasmid before you request it. Full plasmid sequence is available only if provided by the depositing laboratory.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 28287" in your Materials and Methods section.