Addgene - pRSI9-U6-(sh)-UbiC-TagRFP-2A-Puro Plasmid Data

Plasmid 28289: pRSI9-U6-(sh)-UbiC-TagRFP-2A-Puro

Gene/insert name:
None
Alt name:
DECIPHER shRNA Expression Vector
Vector backbone:
pRSI9-U6-(sh)-UbiC-TagRFP-2A-Puro
(Search Vector Database)
Backbone manufacturer:
Cellecta
Vector type:
Mammalian Expression, Lentiviral, RNAi
Backbone size (bp):
7500
Cloning site 5':
BbsI (BpiI)
Site destroyed during cloning:
No
Cloning site 3':
BbsI (BpiI)
Site destroyed during cloning:
No
5' sequencing primer:
FwdU6 (CAAGGCTGTTAGAGAGATAATTGGAA) List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
SURE
Growth temperature (℃):
37
Growth instructions:
Plasmids need to be grown in OmniMAX 2 T1R (Life Technologies) or SURE (Agilent) Recombination defective E.coli cells
High or low copy:
Unknown
Selectable markers:
Puromycin
Sequence: View sequences (3)
Map:
View map
Map:
View map
Supplemental document:
DECIPHER Manual for shRNA cloning (application/pdf)
Supplemental document:
DECIPHER Project Website
Supplemental document:
DECIPHER Data and Pathway Analysis Software
Supplemental document:
DECIPHER Resources (includes link to User Manual)
Principal Investigator:
Alex Chenchik
Principal Investigator:
Gus Frangou
Terms and Licenses:
MTA
Agilent Technologies Inc End User Label License for the use of Cellecta shRNA libraries comprising Oligo Pools
Cellecta Lentiviral Pooled Plasmid and Packaged shRNA Libraries Limited Use Label License
Evrogen IP JSC End User Label License for the use of Cellecta lentiviral shRNA constructs comprising TagRFP-encoded gene
Life Technologies Corporation End-User Label License for the use of Cellecta Lentiviral Expression System

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 28289" in your Materials and Methods section.