Addgene - pCDNA3.1(+) GFP-PIPKI{gamma}661-FLAG Plasmid Data

Plasmid 29583: pCDNA3.1(+) GFP-PIPKI{gamma}661-FLAG

Gene/insert name:
phosphatidylinositol phosphate kinase type I gamma
Alt name:
PIPKI gamma 661
Insert size:
2715
Species:
M. musculus (mouse)
Fusion protein or tag:
GFP
Terminal:
N terminal on insert
Fusion protein or tag:
Flag
Terminal:
C terminal on insert
Mutation:
N/A
Vector backbone:
pCDNA3.1(+)
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5424
Cloning site 5':
BamH1
Site destroyed during cloning:
No
Cloning site 3':
Xho1
Site destroyed during cloning:
No
5' sequencing primer:
CMV-F List of Sequencing Primers
3' sequencing primer:
BGH-rev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
DH5 alpha
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
Ling et al., 2002 from Dr. Richard Anderson
Sequence: View sequences (4)
Principal Investigator:
Anna Huttenlocher
Terms and Licenses:
MTA

Comments:

Please note that Addgene's sequencing results identified a single nucleotide mismatch at bp#440 when compared to the full sequence information provided by the depositing laboratory.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Type Igamma PIP kinase is a novel uropod component that regulates rear retraction during neutrophil chemotaxis. Lokuta et al (Mol Biol Cell. 2007 Dec . 18(12):5069-80. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 29583" in your Materials and Methods section.