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pCDNA3.1(+) GFP-PIPKI{gamma}661-FLAG
(Plasmid #29583)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 29583 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCDNA3.1(+)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5424
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5 alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    phosphatidylinositol phosphate kinase type I gamma
  • Alt name
    PIPKI gamma 661
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2715
  • Mutation
    N/A
  • Tags / Fusion Proteins
    • GFP (N terminal on insert)
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site Xho1 (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Ling et al., 2002 from Dr. Richard Anderson

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that Addgene's sequencing results identified a single nucleotide mismatch at bp#440 when compared to the full sequence information provided by the depositing laboratory.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3.1(+) GFP-PIPKI{gamma}661-FLAG was a gift from Anna Huttenlocher (Addgene plasmid # 29583 ; http://n2t.net/addgene:29583 ; RRID:Addgene_29583)
  • For your References section:

    Type Igamma PIP kinase is a novel uropod component that regulates rear retraction during neutrophil chemotaxis. Lokuta MA, Senetar MA, Bennin DA, Nuzzi PA, Chan KT, Ott VL, Huttenlocher A. Mol Biol Cell. 2007 Dec . 18(12):5069-80. 10.1091/mbc.e07-05-0428 PubMed 17928408