Addgene - pLenti PGK BirA Hygro (w874-1) Plasmid Data

Plasmid 29649: pLenti PGK BirA Hygro (w874-1)

Gene/insert name:
BirA
Insert size:
980
Species:
E.coli
Vector backbone:
p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral
Backbone size w/o insert (bp):
8683
Cloning site 5':
Bam HI
Site destroyed during cloning:
No
Cloning site 3':
Xba I
Site destroyed during cloning:
No
5' sequencing primer:
PGKforw List of Sequencing Primers
3' sequencing primer:
WPRErev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
Stbl3, 37oC
High or low copy:
High Copy
Selectable markers:
Hygromycin
Sequence: View sequences (2)
Map:
View map
Supplemental document:
Notes from Addgene (1)
Principal Investigator:
Eric Campeau
Terms and Licenses:
MTA

Comments:

The BirA gene was amplified from the E.coli TOP10F' strain, sequence confirmed, and functionally tested in E.coli using a protein fused to an AviTag.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 29649" in your Materials and Methods section.