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pTH644-CENBEVY
(Plasmid #29695)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 29695 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRS316
  • Backbone size w/o insert (bp) 4817
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    bidirectional promoter cassette from pBEVY-U
  • Alt name
    P_ADH1/ P_TDH3 cassette
  • Insert Size (bp)
    1797

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer M13f (-40)
  • 3′ sequencing primer M13r (-48)
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pBEVY-U obtained from EUROSCARF collection

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Miller, CA, Martinat, MA, Hyman, LE (1998) Nucleic Acids Res 26(15) 3577-3583. doi: 10.1093/nar/26.15.3577

Please note that there are minor mismatches between depositor's reference sequence and Addgene's quality control sequence. All differences are outside of the cloned gene, and do not affect the function of the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTH644-CENBEVY was a gift from Tobias von der Haar (Addgene plasmid # 29695 ; http://n2t.net/addgene:29695 ; RRID:Addgene_29695)
  • For your References section:

    The role of tRNA and ribosome competition in coupling the expression of different mRNAs in Saccharomyces cerevisiae. Chu D, Barnes DJ, von der Haar T. Nucleic Acids Res. 2011 May 9. ():. 10.1093/nar/gkr300 PubMed 21558172