Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||39207||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7800
Vector typeYeast Expression
Growth in Bacteria
Copy numberLow Copy
SpeciesS. cerevisiae (budding yeast)
Insert Size (bp)11000
Entrez GeneTOR2 (a.k.a. YKL203C, DRR2)
- Promoter GAL
/ Fusion Protein
- HA (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (destroyed during cloning)
- 3′ cloning site SphI (not destroyed)
- 5′ sequencing primer GAL1 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
N-terminally HA-tagged TOR2 expressed under control of GAL promoter.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSEYC68-HA-TOR2 (pAS21) was a gift from Michael Hall (Addgene plasmid # 39207 ; http://n2t.net/addgene:39207 ; RRID:Addgene_39207)
For your References section:HEAT repeats mediate plasma membrane localization of Tor2p in yeast. Kunz J, Schneider U, Howald I, Schmidt A, Hall MN. J Biol Chem. 2000 Nov 24;275(47):37011-20. 10.1074/jbc.M007296200 PubMed 10973982
Map uploaded by the depositor.