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pSEYC68-HA-TOR2 (pAS21)
(Plasmid #39207)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 39207 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSEYC68
  • Backbone size w/o insert (bp) 7800
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    TOR2
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    11000
  • Entrez Gene
    TOR2 (a.k.a. YKL203C, DRR2)
  • Promoter GAL
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (destroyed during cloning)
  • 3′ cloning site SphI (not destroyed)
  • 5′ sequencing primer GAL1
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Depositor Comments

N-terminally HA-tagged TOR2 expressed under control of GAL promoter.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSEYC68-HA-TOR2 (pAS21) was a gift from Michael Hall (Addgene plasmid # 39207 ; http://n2t.net/addgene:39207 ; RRID:Addgene_39207)
  • For your References section:

    HEAT repeats mediate plasma membrane localization of Tor2p in yeast. Kunz J, Schneider U, Howald I, Schmidt A, Hall MN. J Biol Chem. 2000 Nov 24;275(47):37011-20. 10.1074/jbc.M007296200 PubMed 10973982