|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||30216||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2400
Vector typeGateway Entry plasmid
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameNo gene
Insert Size (bp)2700
/ Fusion Proteins
- Flag (C terminal on backbone)
- Bio (C terminal on backbone)
- Cloning method Gateway Cloning
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
pENTR3c was re-engineered to allow fusion of flag-bio to the c-terminus of an ORF. The ORF is cloned in 5'-SalI-EcoRI-3'. The ccdb gene was removed. The fusion construct is transferred to a gateway destination vector by LR clonase.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pENTR-flbio was a gift from William Pu (Addgene plasmid # 30216 ; http://n2t.net/addgene:30216 ; RRID:Addgene_30216)
For your References section:Co-occupancy by multiple cardiac transcription factors identifies transcriptional enhancers active in heart. He A, Kong SW, Ma Q, Pu WT. Proc Natl Acad Sci U S A. 2011 Apr 5. 108(14):5632-7. 10.1073/pnas.1016959108 PubMed 21415370