|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||31070||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backboneModified pEGFP-N1 (see note)
- Backbone size w/o insert (bp) 4000
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneSPTBN1 (a.k.a. ELF, HEL102, SPTB2, betaSpII)
/ Fusion Protein
- HA (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-Fwd (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Articles Citing this Plasmid
This construct DOES NOT contain EGFP- the EGFP has been removed from the pEGFP-N1 backbone.
The following "mutations" have been located in this construct. The Bennett lab believes they are due to incorrect sequence data originally entered into NCBI; however, please be aware of these in case you are designing primers or doing mutagenesis:
Derivations from published sequence:
2.59 c-->t (silent)
4.23 c-->g (H-->D)
5.99 g-->c (R-->T)
6.3 g-->a (silent)
Please refer to the Addgene NGS result for the fully validated sequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:beta2-spectrin-HA was a gift from Vann Bennett (Addgene plasmid # 31070 ; http://n2t.net/addgene:31070 ; RRID:Addgene_31070)