Addgene - pCS_FLPER(T) Plasmid Data

Plasmid 31131: pCS_FLPER(T)

Gene/insert name:
FLP-ER ligand binding domain
Insert size:
2300
Species:
H. sapiens (human), S. cerevisiae (budding yeast)
Mutation:
full length FLP linked to ligand binding domain of estrogen receptor starting at AA 251. Contains V400G and G521R.
Vector backbone:
pCS2+
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4100
Cloning site 5':
BamHI
Site destroyed during cloning:
Yes
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
Gerhart Ryffel
Terms and Licenses:
MTA

Comments:

tamoxifene regulated FLP activity

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: FLP and Cre recombinase function in Xenopus embryos. Werdien et al (Nucleic Acids Res. 2001 Jun 1. 29(11):E53-3. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 31131" in your Materials and Methods section.