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Plasmid 31200: pDONR223-K-RAS V12
  • K-Ras

  • 570

  • H. sapiens (human)

  • KRAS (C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, KRAS2, NS, NS3, RASK2)

  • G12V constitutively active

  • pDONR223
    (Search Vector Database)

  • Invitrogen

  • Gateway Donor vector

  • 5005

  • Gateway Cloning

  • M13pUC-fwd List of Sequencing Primers

  • M13rev

  • Spectinomycin

  • DH5alpha

  • 37

  • High Copy

  • View sequences (1)
  • David Root

  • UBMTA

Comments: 

Do NOT use this plasmid directly for transfections, you will need to recombine it into a destination vector.

A set of 4 custom lentiviral vectors is available (http://www.addgene.org/25895, http://www.addgene.org/25896, http://www.addgene.org/25897, http://www.addgene.org/25890) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A public genome-scale lentiviral expression library of human ORFs. Yang et al (Nat Methods. 2011 Jun 26. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 31200" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only