Addgene - pDONR223-MEKDD Plasmid Data

Plasmid 31202: pDONR223-MEKDD

Gene/insert name:
MAPKK1
Insert size:
1200
Species:
H. sapiens (human)
Entrez Gene:
MAP2K1 (MAPKK1, MEK1, MKK1, PRKMK1)
Mutation:
S218D S222D
Vector backbone:
pDONR223
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Gateway Donor vector
Backbone size w/o insert (bp):
5005
Cloning method:
Gateway Cloning
5' sequencing primer:
M13pUC-fwd List of Sequencing Primers
3' sequencing primer:
M13rev
Bacterial resistance(s)
Spectinomycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
David Root
Terms and Licenses:
MTA

Comments:

The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections.

A set of 4 custom lentiviral vectors is available (http://www.addgene.org/25895, http://www.addgene.org/25896, http://www.addgene.org/25897, http://www.addgene.org/25890) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A public genome-scale lentiviral expression library of human ORFs. Yang et al (Nat Methods. 2011 Jun 26. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 31202" in your Materials and Methods section.