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Plasmid 31210: pLenti6/V5-DEST-HSF1
  • HSF1

  • 1590

  • H. sapiens (human)

  • BC014638

  • HSF1 (HSTF1)

  • V5

  • C terminal on backbone

  • pLenti6/V5-DEST
    (Search Vector Database)

  • Invitrogen

  • Mammalian Expression, Lentiviral

  • 7000

  • Gateway Cloning

  • attR1

  • Yes

  • attR2

  • Yes

  • CMV Forward List of Sequencing Primers

  • V5 Reverse

  • Ampicillin

  • Stbl3

  • 30

  • Grow in STBL3 bacteria (Invitrogen) at lower temps (~30C) to prevent recombination and increase plasmid production.

  • High Copy

  • Blasticidin

  • The entry clone used to create this plasmid was obatined from the human ORFeome (v5.1) collection made available by the Center for Cancer Systems Biology at the Dana-Farber Cancer Institute in Boston, MA.

  • View sequences (1)
  • Lynda Chin

  • UBMTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Proinvasion metastasis drivers in early-stage melanoma are oncogenes. Scott et al (Cancer Cell. 2011 Jul 12;20(1):92-103. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 31210" in your Materials and Methods section.