|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||32537||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5400
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Mutationsee depositor comments below
Entrez GeneHSF1 (a.k.a. HSTF1)
- Promoter CMV
/ Fusion Protein
- FLAG (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site None (unknown if destroyed)
- 3′ cloning site None (unknown if destroyed)
- 5′ sequencing primer CMV-Fwd (Common Sequencing Primers)
Please note that mutation P448L in HSF1 was found during Addgene's quality control. This plasmid should function as reported in the associated publication.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:FLAG-HSF1 was a gift from Stuart Calderwood (Addgene plasmid # 32537 ; http://n2t.net/addgene:32537 ; RRID:Addgene_32537)
For your References section:Regulation of molecular chaperone gene transcription involves the serine phosphorylation, 14-3-3 epsilon binding, and cytoplasmic sequestration of heat shock factor 1. Wang X, Grammatikakis N, Siganou A, Calderwood SK. Mol Cell Biol. 2003 Sep;23(17):6013-26. 10.1128/MCB.23.17.6013-6026.2003 PubMed 12917326