Addgene - pBP2MF Plasmid Data

Plasmid 31279: pBP2MF

Gene/insert name:
Per2
Insert size:
3831
Species:
M. musculus (mouse)
Entrez Gene:
Per2 (mKIAA0347, mPer2)
Fusion protein or tag:
Myc
Terminal:
N terminal on insert
Fusion protein or tag:
Flag
Terminal:
C terminal on insert
Vector backbone:
pFastBac1
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Insect Expression
Backbone size w/o insert (bp):
4775
Cloning site 5':
SpeI
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Gentamicin
Sequence: View sequences (2)
Principal Investigator:
Aziz Sancar
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Biochemical analysis of the canonical model for the mammalian circadian clock. Ye et al (J Biol Chem. 2011 May 27. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 31279" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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