Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||31309||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3000
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namecardiac actin / Cre
SpeciesX. laevis (frog); bateriophage P1
Insert Size (bp)4500
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
pCARGFP (Kroll et al 1996) used to replace the GFP with Cre from pCSCre2
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCARCre was a gift from Gerhart Ryffel (Addgene plasmid # 31309 ; http://n2t.net/addgene:31309 ; RRID:Addgene_31309)
For your References section:Tagging muscle cell lineages in development and tail regeneration using Cre recombinase in transgenic Xenopus. Ryffel GU, Werdien D, Turan G, Gerhards A, Goosses S, Senkel S. Nucleic Acids Res. 2003 Apr 15. 31(8):e44. 10.1093/nar/gng044 PubMed 12682379
Map uploaded by the depositor.