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pCARCre
(Plasmid #31309)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 31309 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cardiac actin / Cre
  • Species
    X. laevis (frog); bateriophage P1
  • Insert Size (bp)
    4500

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

pCARGFP (Kroll et al 1996) used to replace the GFP with Cre from pCSCre2

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCARCre was a gift from Gerhart Ryffel (Addgene plasmid # 31309 ; http://n2t.net/addgene:31309 ; RRID:Addgene_31309)
  • For your References section:

    Tagging muscle cell lineages in development and tail regeneration using Cre recombinase in transgenic Xenopus. Ryffel GU, Werdien D, Turan G, Gerhards A, Goosses S, Senkel S. Nucleic Acids Res. 2003 Apr 15. 31(8):e44. 10.1093/nar/gng044 PubMed 12682379