1
Plasmid 31467: pTRETightBI-RY-1pf
  • eYFP and mCherry

  • 740

  • fluorescent proteins

  • NLS

  • N terminal on insert

  • pTRETightBI
    (Search Vector Database)

  • Clontech

  • Mammalian Expression

  • 2850

  • EcoRI, BamHI

  • No

  • NdeI, ClaI

  • No

  • n/a List of Sequencing Primers

  • Ampicillin

  • Top10

  • 30

  • Grow TopTen cells at 30C instead of 37C to reduce risk of recombination at the SV40 pA sites.

  • Unknown

  • View sequences (2)
  • Phil Sharp

  • UBMTA
    Clontech Limited Use Label License
    Ancillary Agreement for Plasmids Containing FP Materials

Comments: 

1pf = 1 site for miR-20 with perfect (pf) complementarity to the miRNA in mCherry 3'UTR

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: MicroRNAs can generate thresholds in target gene expression. Mukherji et al (Nat Genet. 2011 Aug 21;43(9):854-9. doi: 10.1038/ng.905. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 31467" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only