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pTRETightBI-RY-4
(Plasmid #31465)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 31465 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pTRETightBI
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 2850
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Top10
  • Growth instructions
    Grow TopTen cells at 30C instead of 37C to reduce risk of recombination at the SV40 pA sites.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    eYFP and mCherry
  • Species
    fluorescent proteins
  • Insert Size (bp)
    740
  • Tag / Fusion Protein
    • NLS (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI, BamHI (not destroyed)
  • 3′ cloning site NdeI, ClaI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

4 tandem miR-20 binding sites in mCherry 3'UTR

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTRETightBI-RY-4 was a gift from Phil Sharp (Addgene plasmid # 31465 ; http://n2t.net/addgene:31465 ; RRID:Addgene_31465)
  • For your References section:

    MicroRNAs can generate thresholds in target gene expression. Mukherji S, Ebert MS, Zheng GX, Tsang JS, Sharp PA, van Oudenaarden A. Nat Genet. 2011 Aug 21;43(9):854-9. doi: 10.1038/ng.905. 10.1038/ng.905 PubMed 21857679