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pLS1/-11
(Plasmid #31492)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 31492 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Lactose repressor with 11 amino acid C-terminal deletion
  • Alt name
    LacI/-11aa
  • Alt name
    LacI-11
  • Species
    E. coli variant
  • Insert Size (bp)
    2970
  • Entrez Gene
    lacI (a.k.a. b0345, ECK0342)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site EcoR1 (destroyed during cloning)
  • 5′ sequencing primer CCAGCCAGCCAGACGCAG
  • 3′ sequencing primer CTGGGCGTGGAGCATCTG
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid is utilized to produce dimeric lactose repressor protein.

For Expression: E. coli BLIM cells or any E. coli WITHOUT LacI in the genome or another plasmid

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLS1/-11 was a gift from Kathleen Matthews (Addgene plasmid # 31492 ; http://n2t.net/addgene:31492 ; RRID:Addgene_31492)