pLS1/-11
(Plasmid #31492)

• Depositing Lab: Kathleen Matthews
• Publication: pLS/-11 combination of pLS1 encoding lactose repressor with deletion of 11 C-terminal amino acids (unpublished)

Backbone

• Vector backbone: pLS1
• Backbone size w/o insert (bp): 4500
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Lactose repressor with 11 amino acid C-terminal deletion
• Alt name: LacI/-11aa
• Alt name: LacI-11
• Species: E. coli variant
• Insert Size (bp): 2970
• Entrez Gene: lacI (a.k.a. b0345, ECK0342)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (not destroyed)
• 3′ cloning site: EcoR1 (destroyed during cloning)
• 5′ sequencing primer: CCAGCCAGCCAGACGCAG
• 3′ sequencing primer: CTGGGCGTGGAGCATCTG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is utilized to produce dimeric lactose repressor protein.

For Expression: E. coli BLIM cells or any E. coli WITHOUT LacI in the genome or another plasmid