pcDNA3-YFP-PKD1
(Plasmid #31527)

• Depositing Lab: Alexandra Newton
• Publication: Kunkel et al J Biol Chem. 2009 Sep 4. 284(36):24653-61.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: YFP-HA-Protein Kinase D
• Alt name: YFP-PKD
• Alt name: PKD
• Species: H. sapiens (human)
• Entrez Gene: PRKD1 (a.k.a. CHDED, PKC-MU, PKCM, PKD, PRKCM)
• Tags / Fusion Proteins:
  • YFP (N terminal on insert)
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH1 (not destroyed)
• 3′ cloning site: Xho1 (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: Sp6

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The HA tag on this plasmid contains a point mutation that renders it unrecognizable by the 3F10 HA antibody. It should work fine with the 12CA5 HA antibody.