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pHA-ΔN331-Kin1B
(Plasmid #31603)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 31603 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    E. coli DH5α, 16hrs at 37C
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ΔN331 kinesin-1B
  • Alt name
    headless kinesin-1B
  • Alt name
    headless KIF5B
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2168
  • Mutation
    deleted amino acids 1-331
  • GenBank ID
    BC090841
  • Entrez Gene
    Kif5b (a.k.a. AL022807, Khc, Khcs, Kns1, Ukhc)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA-tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer specific sequence primer
  • 3′ sequencing primer specific sequence primer
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    I.M.A.G.E. clone 30543821 obtained from Open Biosystems
  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHA-ΔN331-Kin1B was a gift from Anthony Brown (Addgene plasmid # 31603)
  • For your References section:

    Tight functional coupling of kinesin-1A and dynein motors in the bidirectional transport of neurofilaments. Uchida A, Alami NH, Brown A. Mol Biol Cell. 2009 Dec . 20(23):4997-5006. 10.1091/mbc.E09-04-0304 PubMed 19812246