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pBMN YFP-FKBP(DD)
(Plasmid #31766)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 31766 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBMN
  • Backbone manufacturer
    Garry Nolan
  • Backbone size w/o insert (bp) 6943
  • Vector type
    Retroviral
  • Selectable markers
    HcRed fluorescence behind IRES

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Top10/P3
  • Growth instructions
    Culture at 30C to prevent recombination of LTR and lost insert. Takes >20 hours to get good culture at 30C
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    HA-YFP-FKBP(DD)
  • Alt name
    YFP and FKBP12 (destable domain)
  • Alt name
    YFP fused with FK506 binding protein 1A( destable domain)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1095
  • Mutation
    E31G, F36V, R71G and K105E on FKBP12
  • GenBank ID
    NM_000801
  • Promoter LTR
  • Tags / Fusion Proteins
    • YFP (N terminal on insert)
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer pBMN vector specific
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBMN YFP-FKBP(DD) was a gift from Thomas Wandless (Addgene plasmid # 31766)
  • For your References section:

    Recent progress with FKBP-derived destabilizing domains. Chu BW, Banaszynski LA, Chen LC, Wandless TJ. Bioorg Med Chem Lett. 2008 Nov 15;18(22):5941-4. Epub 2008 Sep 12. 10.1016/j.bmcl.2008.09.043 PubMed 18815033