Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||31819||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5600
Modifications to backboneThe minimal c-fos promoter fragment was cloned into the SmaI and BglII sites of pGL2 basic.
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameMutant MEF2-dependent promoter (-307 to -242 of Nur77/NR4A1)
SpeciesM. musculus (mouse)
Mutationtwo CTATATTTAG RSRF sites mutated to CGATATTTCG
- Promoter mutated MEF2-dependent (-307 to -242 of Nur77/NR4A1)
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (unknown if destroyed)
- 3′ cloning site SalI (unknown if destroyed)
- 5′ sequencing primer EBV-reverse
- 3′ sequencing primer LucNrev (Common Sequencing Primers)
The minimal c-fos promoter fragment was cloned into the SmaI and BglII sites of pGL2 basic. The mutated RSRF sequence (two CTATATTTAG RSRF sites mutated to CGATATTTCG) of the -307 to -242 Nur77/NR4A1 promoter region was cloned in to the SalI site of this SmaI/BglII fragment.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:RSRF-Luc-2mut was a gift from Astar Winoto (Addgene plasmid # 31819 ; http://n2t.net/addgene:31819 ; RRID:Addgene_31819)
For your References section:Regulation of the Nur77 orphan steroid receptor in activation-induced apoptosis. Woronicz JD, Lina A, Calnan BJ, Szychowski S, Cheng L, Winoto A. Mol Cell Biol. 1995 Nov;15(11):6364-76. PubMed 7565789