Addgene - pCR4-24XPP7SL Plasmid Data

Plasmid 31864: pCR4-24XPP7SL

Gene/insert name:
24xPP7 Stem Loop Cassette
Insert size:
1450
Vector backbone:
pCR4blunt
(Search Vector Database)
Cloning method:
TOPO Cloning
5' sequencing primer:
M13R List of Sequencing Primers
3' sequencing primer:
M13F
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Supplemental document:
Notes from Addgene (1)
Principal Investigator:
Robert Singer
Terms and Licenses:
MTA

Comments:

The 24xPP7 stem loop cassettes in this plasmid were generated from two non-identical stem-loops to reduce unnecessary redundancy and the chance of recombination in bacteria. The sequence for a single cassette (with the two non-identical stem-loops in uppercase) is:

taaggtacctaattgcctagaaaGGAGCAGACGATATGGCGTCGCTCCctgcaggtcgactctagaaa- CCAGCAGAGCATATGGGCTCGCTGGctgcagtattcccgggttcatt

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Real-time observation of transcription initiation and elongation on an endogenous yeast gene. Larson et al (Science. 2011 Apr 22;332(6028):475-8. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 31864" in your Materials and Methods section.