Addgene - pET22HT-PP7delFG Plasmid Data

Plasmid 31863: pET22HT-PP7delFG

Gene/insert name:
PP7delFG Coat Protein
GenBank ID:
2QUD
Fusion protein or tag:
His6-tag
Terminal:
N terminal on insert
Fusion protein or tag:
TEV site
Terminal:
N terminal on insert
Mutation:
deleted FG loop
Vector backbone:
pET22
(Search Vector Database)
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Robert Singer
Terms and Licenses:
MTA
EMD Millipore Limited Use Label License/Brookhaven pET Assurance Letter

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Structural basis for the coevolution of a viral RNA-protein complex. Chao et al (Nat Struct Mol Biol. 2008 Jan;15(1):103-5. Epub 2007 Dec 9. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 31863" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

31864: pCR4-24XPP7SL

31865: pCR4-24XMS2SL-stable

27121: pMS2-GFP

27122: pMS2-YFP

27119: pSL-MS2-12X