pET22HT-PP7delFG
(Plasmid #31863)

Available to Academic and Nonprofits Only

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    PP7delFG Coat Protein
  • Mutation
    deleted FG loop
  • GenBank ID
    2QUD
  • Tags / Fusion Proteins
    • His6-tag (N terminal on insert)
    • TEV site (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET22HT-PP7delFG was a gift from Robert Singer (Addgene plasmid # 31863)
  • For your References section:

    Structural basis for the coevolution of a viral RNA-protein complex. Chao JA, Patskovsky Y, Almo SC, Singer RH. Nat Struct Mol Biol. 2008 Jan;15(1):103-5. Epub 2007 Dec 9. 10.1038/nsmb1327 PubMed 18066080